Antibacterial Potential and Phytochemical Profiling of Lawsonia Inermis Ethanolic Extract against Skin-Associated Bacterial Pathogens
Effect of Lawsonia Inermis against Skin Pathogens
Keywords:
Antibacterial Agent, Antibiotic, Lawsonia Inermis, Phytochemicals, Pathogens, Skin DiseasesAbstract
Lawsonia inermis (henna) has long been recognized in traditional medicine for its bioactive nature, particularly its antimicrobial and anti-inflammatory effects. These properties highlight its potential as a natural therapeutic agent for wound infection control and healing, mitigating inflammation and soothing diverse skin conditions. Considering its medicinal attributes L. inermis, was used in the current study as a natural agent for the control of skin infections causing pathogens. Notable bacterial pathogens associated with skin diseases include Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The ethanolic extract of L. inermis extract exhibiting alkaloids, saponins, phenols, steroids, terpenoids, anthraquinones and tannins. The antibiotic susceptibility test revealed that most bacterial species were sensitive to commonly used antibiotics. Bacterial cultures revealed a dose dependent relationship using the agar well diffusion assay. Isolates of S. aureus displayed the largest zone (22 ± 0.81 mm) at a concentration of 1.5 mg/ml followed by P. aeruginosa (21 ± 0.81 mm) and E. coli (17 ± 1.63 mm). The L. inermis extract displayed MIC of 0.375 mg/ml for ATCC and clinical isolates of E. coli and S. aureus, and 0.188 mg/ml for P. aeruginosa strains, with MBC of 0.75 mg/ml recorded for all tested strains. These findings indicate that ethanolic L. inermis extract has promising in-vitro antibacterial activity against selected skin-associated bacterial pathogens and further suggests its potential as a natural agent for combating skin infections.
DOI: https://doi.org/10.59564/amrj/04.04/002
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